The Emerin/EMD Antibody (CAB13925) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody is produced in rabbits and has been validated for use in various applications, including Western blot analysis. It specifically binds to the EMD protein, allowing for accurate detection and analysis in a variety of cell types.EMD, also known as Emerin, plays a significant role in maintaining the integrity of the nuclear envelope and is implicated in the regulation of gene expression and signal transduction. Dysregulation of EMD has been linked to a range of diseases, including Emery-Dreifuss muscular dystrophy and certain forms of cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Emerin/EMD Antibody
SKU:
CAB13925
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Purification Method
Affinity purification
Gene ID
2010
RRID
AB_2760777
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Emerin/EMD Rabbit pAb (CAB13925) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Emerin/EMD Rabbit pAb (CAB13925) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using Emerin/EMD Rabbit pAb (CAB13925) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using Emerin/EMD Rabbit pAb (CAB13925) at dilution of 1:100. Blue: DAPI for nuclear staining.
