The EMX2 Polyclonal Antibody (PAC054754) is a valuable tool for researchers interested in studying EMX2, a homeobox transcription factor known for its role in brain development and tumorigenesis. This antibody, raised in rabbits, is highly specific to human EMX2 and has been validated for use in Western blot and immunohistochemistry applications.EMX2 is a key player in regulating the differentiation and survival of neural stem cells, making it a crucial factor in brain development.
Additionally, dysregulation of EMX2 has been linked to tumorigenesis in various types of cancer, highlighting its potential as a therapeutic target. By using the EMX2 Polyclonal Antibody, researchers can investigate the expression and function of EMX2 in different cell types and tissues, providing valuable insights into its role in both normal development and disease progression.
Western Blot. Positive WB detected in: Rat brain tissue, Mouse brain tissue. All lanes: EMX2 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 19 kDa. Observed band size: 29 kDa.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO54754 at dilution of 1:100.
Background:
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Synonyms:
Homeobox protein EMX2 (Empty spiracles homolog 2) (Empty spiracles-like protein 2), EMX2
UniProt Protein Function:
EMX2: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Defects in EMX2 are the cause of schizencephaly (SCHZC). Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro- spinal fluid. Belongs to the EMX homeobox family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 10q26.1Cellular Component: nucleusMolecular Function: protein binding; sequence-specific DNA bindingBiological Process: forebrain cell migration; neuron differentiation; response to drug; anterior/posterior pattern formation; dentate gyrus development; regulation of transcription, DNA-dependent; cell proliferation in forebrain; cerebral cortex regionalizationDisease: Schizencephaly
UniProt Protein Details:
NCBI Summary:
This gene encodes a homeobox-containing transcription factor that is the homolog to the 'empty spiracles' gene in Drosophila. Research on this gene in humans has focused on its expression in three tissues: dorsal telencephalon, olfactory neuroepithelium, and urogenetial system. It is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and adult olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10. Alternative splicing results in multiple transcript variants encoding distinct proteins.[provided by RefSeq, Sep 2009]
