The ENO2 Antibody (CAB12341) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.Gamma-enolase is a multifunctional protein that plays a crucial role in various biological processes, including metabolism and neurodevelopment. Its dysregulation has been linked to a range of diseases, such as cancer, neurodegenerative disorders, and autoimmune conditions.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ENO2 Antibody
SKU:
CAB12341
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NSE, HEL-S-279, ENO2
Positive Sample:
SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Purification Method
Affinity purification
Gene ID
2026
RRID
AB_2759186
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ENO2 Rabbit pAb (CAB12341) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using ENO2 Rabbit pAb (CAB12341) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Raji cells using ENO2 Rabbit pAb (CAB12341) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg ENO2 antibody (CAB12341). Western blot was performed from the immunoprecipitate using ENO2 antibody (CAB12341) at a dilution of 1:1000.
