The ErbB4/HER4 Monoclonal Antibody (CAB19047) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to ERBB4, this antibody enables precise detection and analysis of the protein in different cell types, making it a valuable asset for studies in cancer biology and signaling cascades.ERBB4, also known as HER4, is a member of the epidermal growth factor receptor family and is associated with cell proliferation, differentiation, and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ErbB4/HER4 Monoclonal Antibody
SKU:
CAB19047
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2639
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HER4, ALS19, p180erbB4, ErbB4/HER4
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Mitochondrion, Nucleus, Single-Pass Type I Membrane Protein.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Purification Method
Affinity purification
Gene ID
2066
RRID
AB_2862540
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ErbB4/HER4 Rabbit mAb (CAB19047) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
