The ERCC3 Antibody (CAB12702) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the ERCC3 protein, this antibody enables researchers to detect and analyze the expression levels of ERCC3 in various cell types.ERCC3, also known as Xeroderma pigmentosum group B-complementing protein, is involved in the nucleotide excision repair pathway, which helps to remove DNA damage caused by UV radiation and other environmental factors.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ERCC3 Antibody
SKU:
CAB12702
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
XPB, BTF2, Ssl2, TTD2, GTF2H, RAD25, TFIIH, ERCC3
Positive Sample:
Jurkat, Mouse kidney, Rat kidney
Cellular Localization:
Nucleus.
Calculated MW:
89kDa
Observed MW:
89kDa
This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2071
RRID
AB_2759545
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ERCC3 Rabbit pAb (CAB12702) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
