The EXT1 Antibody (CAB2030) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to Exostosin-1, this antibody allows for the detection and analysis of the protein in various cell types, making it an essential component for studies in glycobiology and cancer research.Exostosin-1 is involved in the process of heparan sulfate proteoglycan biosynthesis, playing a crucial role in cell growth, differentiation, and signaling.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EXT1 Antibody
SKU:
CAB2030
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EXT, LGS, TTV, LGCR, TRPS2, EXT1
Positive Sample:
K-562, SW480, PC-3, Mouse liver, Mouse lung
Cellular Localization:
Endoplasmic Reticulum Membrane, Golgi Apparatus Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
86kDa
Observed MW:
86kDa
This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses.
Purification Method
Affinity purification
Gene ID
2131
RRID
AB_2764054
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EXT1 Rabbit pAb (CAB2030) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using EXT1 Rabbit pAb (CAB2030) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using EXT1 Rabbit pAb (CAB2030) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using EXT1 Rabbit pAb (CAB2030) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
