The Ezrin Monoclonal Antibody (CAB19048) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly specific antibody, produced in rabbits, is optimized for use in a variety of applications, including Western blotting and immunofluorescence.Ezrin is a critical component of the cytoskeleton and plays a crucial role in cell adhesion, migration, and signaling. Dysregulation of Ezrin has been implicated in various diseases, including cancer and metastasis.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Ezrin Monoclonal Antibody
SKU:
CAB19048
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0392
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Purification Method
Affinity purification
Gene ID
7430
RRID
AB_2862541
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HCT116 cells, using Ezrin Rabbit mAb (CAB19048) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Ezrin Rabbit mAb (CAB19048) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
