The F-TESTO (Free Testosterone) ELISA Kit is meticulously designed for the quantitative detection of Free Testosterone levels in various biological samples. Free Testosterone is an essential hormone involved in numerous biological processes, including muscle growth, bone density, and sex drive.
It has a significant role in maintaining overall health and wellbeing. Accurate detection of Free Testosterone is crucial for understanding its implications in various health conditions, including metabolic disorders, sexual dysfunction, and certain cancers. Assay Genie's F-TESTO ELISA Kit delivers superior sensitivity and specificity, providing reliable and reproducible results. Manufactured under rigorous quality control standards, this kit assures strong performance and is user-friendly, making it an exceptional choice for both research and clinical applications. Trust Assay Genie's F-TESTO ELISA Kit for precise and reliable detection of this important biomarker in your studies.
Product Name:
F-TESTO (Free Testosterone) ELISA Kit
SKU:
AEES00021
Target:
F-TESTO (Free Testosterone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with F-TESTO. During the reaction, F-TESTO in the sample or standard competes with a fixed amount of F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-98
95-106
95-106
Average (%)
93
103
101
1:4
Range (%)
87-99
95-106
87-100
Average (%)
91
101
93
1:8
Range (%)
98-109
87-101
90-104
Average (%)
105
94
97
1:16
Range (%)
96-104
82-95
92-105
Average (%)
100
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
96-107
103
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.04
216.36
884.05
87.82
219.17
800.62
Standard deviation
4.97
10.17
46.59
4.51
10.76
42.43
C V (%)
5.07
4.7
5.27
5.14
4.91
5.3
Sample type &Sample volume:
serum and plasma; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of F-TESTO concentrations in serum and plasma.
Specificity:
This kit recognizes F-TESTO in samples. No significant cross-reactivity or interference between F-TESTO and analogues was observed.
