The F-TESTO (Free Testosterone) ELISA Kit is meticulously designed for the quantitative detection of Free Testosterone levels in various biological samples. Free Testosterone is an essential hormone involved in numerous biological processes, including muscle growth, bone density, and sex drive. It has a significant role in maintaining overall health and wellbeing. Accurate detection of Free Testosterone is crucial for understanding its implications in various health conditions, including metabolic disorders, sexual dysfunction, and certain cancers. Assay Genie's F-TESTO ELISA Kit delivers superior sensitivity and specificity, providing reliable and reproducible results. Manufactured under rigorous quality control standards, this kit assures strong performance and is user-friendly, making it an exceptional choice for both research and clinical applications. Trust Assay Genie's F-TESTO ELISA Kit for precise and reliable detection of this important biomarker in your studies.
Product Name:
F-TESTO (Free Testosterone) ELISA Kit
SKU:
AEES00021
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
