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F4/80 Monoclonal Antibody (CAB23788)

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SKU:
CAB23788
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse Rat
Host Species:
Rabbit
Synonyms:
Emr1 Ly71 F4/80 Gpf480 TM7LN3 DD7A5-7 EGF-TM7
$173 - $603
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Description

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F4/80 Monoclonal Antibody (CAB23788)

The F4/80 Monoclonal Antibody (CAB23788) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).

This antibody is validated for use in IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:F4/80 Monoclonal Antibody
SKU:CAB23788
Size:100μL, 20μL
Reactivity:Mouse, Rat
Clone Number:ARC61555
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:IHC-P ELISA IF-P
Recommended Dilution:
IF-P1:100 - 1:400
IHC-P1:300 - 1:1200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Emr1, Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7
Cellular Localization:Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:102kDa
Observed MW:Refer to figures

Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).

Purification MethodAffinity purification
Gene ID13733
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse thymus tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageConfocal imaging of paraffin-embedded Mouse spleen tissue using F4/80 Rabbit mAb (CAB23788, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Product ImageConfocal imaging of paraffin-embedded Mouse liver tissue using F4/80 Rabbit mAb (CAB23788, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

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