Description
FANCA Monoclonal Antibody (CAB9529)
The FANCA Monoclonal Antibody (CAB9529) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, is highly specific to the FANCA protein and is validated for use in various experimental techniques, including Western blot and immunohistochemistry.FANCA is a key component of the Fanconi anemia pathway, which is responsible for repairing DNA damage and maintaining genomic stability. Mutations in the FANCA gene can disrupt this pathway, leading to the cellular defects and increased cancer susceptibility seen in individuals with Fanconi Anemia.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | FANCA Monoclonal Antibody |
| SKU: | CAB9529 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC1620 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | SDSW VPNS ASGQ DPGG RRRA WAEL LAGR VKRE KYNP ERAQ KLKE SAVR LLRS HQDL NALL LEVE GPLC KKLS LSKV IDCD SSEA YANH SSSF IGSA LQDQ ASR | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | FA, FA1, FAA, FAH, FA-H, FACA, FANCH, FANCA |
| Positive Sample: | HeLa, HT-29, 293T |
| Cellular Localization: | Cytoplasm, Nucleus. |
| Calculated MW: | 163kDa |
| Observed MW: | 163kDa |
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia.
| Purification Method | Affinity purification |
| Gene ID | 2175 |
| RRID | AB_2863715 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using FANCA Rabbit mAb (CAB9529) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
