The FBP2 Antibody (CAB9850) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and is optimized for use in Western blot applications. By binding to the FBP2 protein, it enables researchers to detect and analyze FBP2 expression in various cell types, making it a versatile tool for studies in metabolism and cancer research.FBP2, also known as fructose-1,6-bisphosphatase 2, plays a crucial role in gluconeogenesis, the process by which glucose is synthesized from non-carbohydrate precursors.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
FBP2 Antibody
SKU:
CAB9850
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CORLK, FBP2
Positive Sample:
Mouse liver, Mouse skeletal muscle, Rat skeletal muscle, Rat kidney
Cellular Localization:
Cell Junction, Cytoplasm, Nucleus, Z Line, Myofibril, Sarcomere.
Calculated MW:
37kDa
Observed MW:
36kDa
This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate.
Purification Method
Affinity purification
Gene ID
8789
RRID
AB_2769425
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FBP2 Rabbit pAb (CAB9850) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded Mouse skeletal muscle using FBP2 Rabbit pAb (CAB9850) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.