The FBXO32 Polyclonal Antibody (CAB6825) is a valuable tool for researchers studying FBXO32, a protein involved in regulating muscle atrophy and protein degradation pathways. This antibody, produced in rabbits, is highly specific to FBXO32 in human samples and has been validated for use in Western blot applications. With its ability to detect and analyze FBXO32 expression in various cell types, this antibody is ideal for investigations in muscle biology, metabolism, and cancer research.
FBXO32, also known as atrogin-1, plays a crucial role in controlling protein turnover and muscle wasting in conditions such as cachexia, sarcopenia, and muscle disuse. Its involvement in ubiquitin-mediated proteolysis makes FBXO32 a key target for studying muscle degradation processes and potential therapeutic interventions. By understanding the function of FBXO32, researchers can develop strategies to prevent muscle loss and improve outcomes in muscle-related diseases.
Product Name:
Fbx32/FBOX32 Rabbit Polyclonal Antibody
SKU:
CAB6825
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 206-355 of human Fbx32/FBXO32 (NP_478136.1).
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
114907
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Western blot analysis of lysates from RD cells, using Fbx32/FBXO32 Rabbit pAb (CAB6825) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
