FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (CABS083)
The FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (CABS083) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins. RRID Gene ID Swiss Prot Synonym
This antibody is validated for use in IF/ICC, FC, IF-P applications and has demonstrated reactivity against Rabbit samples.
Product Name:
FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
SKU:
CABS083
Size:
100μL
Reactivity:
Rabbit
Clone Number:
-
Conjugate:
FITC. Ex:491nm. Em:516nm.
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCFCIF-P
Recommended Dilution:
IF
/
ICC
1:100 - 1:500
IF-P
1:100 - 1:500
FC
1:50 - 1:200
Synonyms:
-
Positive Sample:
-
Cellular Localization:
-
Calculated MW:
-
Observed MW:
-
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins. RRID Gene ID Swiss Prot Synonym
Purification Method:
Affinity purification
Gene ID:
-
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(CABS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of SH-SY5Y cells using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(CABS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
