FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (CABS083)
The FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (CABS083) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, targeted against the Fc region of rabbit IgG, allows for specific labeling and detection of rabbit IgG in various applications such as immunofluorescence and flow cytometry.With its high sensitivity and specificity, this antibody is an essential reagent for studies investigating antibody-mediated immune responses and immune cell interactions.
This antibody is validated for use in IF/ICC, FC, IF-P applications and has demonstrated reactivity against Rabbit samples.
Product Name:
FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
SKU:
CABS083
Size:
100μL
Reactivity:
Rabbit
Conjugate:
FITC. Ex:491nm. Em:516nm.
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCFCIF-P
Recommended Dilution:
IF/ICC
1:100 - 1:500
IF-P
1:100 - 1:500
FC
1:50 - 1:200
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (CAB17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(CABS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of SH-SY5Y cells using βIII-Tubulin Rabbit mAb (CAB17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(CABS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
