The FITC-conjugated Goat anti-Rat IgG (H+L) (CABS019) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in IF/ICC, FC applications and has demonstrated reactivity against Rat samples.
Product Name:
FITC-conjugated Goat anti-Rat IgG (H+L)
SKU:
CABS019
Size:
100μL
Reactivity:
Rat
Conjugate:
FITC. Ex:491nm. Em:516nm.
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCFC
Recommended Dilution:
IF/ICC
1:50 - 1:200
FC
1:50 - 1:200
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769477
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Flow cytometry: 1X10^6 RK13 cells (negative control,left) and RK13-CD20 transfection cells (right) were surface-stained with rat anti-mouse CD20 Antibody (1:100,orange line) or secondary antibody only (blue line). Non-fluorescently stained RK13 and RK13 transfection cells were used as blank control (red line). FITC Goat Anti-Rat IgG (H+L)(CABS019, 1:100) was used as a secondary antibody.
