The VEGFR1 Antibody (CAB1277) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been rigorously validated for Western blot applications.FLT1, also known as vascular endothelial growth factor receptor 1 (VEGFR-1), plays a crucial role in regulating blood vessel formation and growth. By binding to FLT1, this antibody allows for the detection and analysis of FLT1 expression in various cell types, enabling researchers to investigate its involvement in processes such as tumor angiogenesis, cardiovascular development, and wound healing.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VEGFR1 Antibody
SKU:
CAB1277
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FLT, FLT-1, VEGFR1, VEGFR-1
Positive Sample:
Mouse lung, Mouse kidney, Rat lung
Cellular Localization:
Cell Membrane, Cytoplasm, Endosome, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
151kDa
Observed MW:
151kDa
This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.
Purification Method
Affinity purification
Gene ID
2321
RRID
AB_2759613
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using VEGFR1 Rabbit pAb (CAB1277) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using VEGFR1 Rabbit pAb (CAB1277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using VEGFR1 Rabbit pAb (CAB1277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
