This ELISA kit is consisted of Micro-plate pre-coated with FMDV-A antibody, Antibody Working Solution, HRP Conjugate, Antigen Solution and other reagents. It applies Inhibition-ELISA as the method for the detection of FMDV-A antibody in Cattle, Goat, Sheep, Porcine samples. Diluted samples and antigen are added into the Micro-plate and incubate. Antibodies in sample will compete with antibody pre-coated on the Micro-plate for the antigen and block the combination between the antigen and the Micro-plate. Then wash and add the Antibody Working Solution to incubate. Wash and add HRP Conjugate, incubate. Then wash away the unbound HRP Conjugate. Add TMB substrate to the wells, it will react with the enzyme and become blue, the shade of color is of negative correlation with the content of specific antibody in the samples. At last, end the reaction by adding Stop Solution to produce a yellow product. Measure the absorbance value of each well by using a Micro-plate Reader with 450 nm wavelength, then the presence of FMDV-A antibody can be determined.
