The fT4 (Free Thyroxine) ELISA Kit is designed for the quantitative detection of Free Thyroxine (fT4) levels in various biological samples. fT4 is a crucial thyroid hormone that plays a significant role in regulating a wide array of physiological activities, including metabolic rate, heart and digestive functions, muscle control, brain development and function, and bone maintenance.
Accurate fT4 measurement is essential for understanding its role in thyroid disorders, metabolic abnormalities, and encephalopathy. Assay Genie's fT4 ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's fT4 ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
fT4 (Free Thyroxine) ELISA Kit
SKU:
AEES00005
Target:
fT4 (Free Thyroxine)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT4. During the reaction, fT4 in the sample or standard competes with a fixed amount of fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to fT4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of fT4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
99-112
91-104
Average (%)
93
106
98
1:4
Range (%)
85-96
92-108
92-105
Average (%)
90
98
100
1:8
Range (%)
88-104
85-98
94-109
Average (%)
95
92
101
1:16
Range (%)
89-106
87-99
96-111
Average (%)
97
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.1
11.0
44.2
5.2
11.0
45.0
Standard deviation
0.3
0.5
2.3
0.3
0.5
2.0
C V (%)
5.88
4.55
5.2
5.77
4.55
4.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of fT4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes fT4 in samples. No significant cross-reactivity or interference between fT4 and analogues was observed.
