The FXN/Frataxin Antibody (CAB11785) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the FXN protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of mitochondrial biology and neurodegenerative diseases.Frataxin is a critical protein that plays a pivotal role in maintaining mitochondrial health and preventing oxidative damage.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FXN/Frataxin Antibody
SKU:
CAB11785
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FA, X25, CyaY, FARR, FRDA, FXN / Frataxin
Positive Sample:
HepG2, 293T, A-549
Cellular Localization:
Cytoplasm, Mitochondrion.
Calculated MW:
23kDa
Observed MW:
19kDa
This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2395
RRID
AB_2758754
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using FXN / Frataxin Rabbit pAb (CAB11785) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using FXN / Frataxin Rabbit pAb (CAB11785) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of L929 cells using FXN / Frataxin Rabbit pAb (CAB11785) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Hep G2 cells using FXN / Frataxin Rabbit pAb(CAB11785) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
