The G6PD Monoclonal Antibody (CAB11234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2861529 Gene ID 2539 Swiss Prot Synonym G6PD1; G6PD
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
G6PD Monoclonal Antibody
SKU:
CAB11234
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0553
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
G6PD1, G6PD
Positive Sample:
HeLa, MCF7
Cellular Localization:
Cytoplasm, Cytoplasmic Side Of Plasma Membrane, Cytosol, Extracellular Exosome, Nucleus.
Calculated MW:
59kDa
Observed MW:
58kDa
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2861529 Gene ID 2539 Swiss Prot Synonym G6PD1; G6PD
Purification Method:
Affinity purification
Gene ID:
2539
RRID:
AB_2861529
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using G6PD Rabbit mAb (CAB11234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
