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Anti-GABARAPL2 Antibody (CAB7782)

SKU:
CAB7782
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
  • Autophagy Antibodies Anti-GABARAPL2 Antibody CAB7782
  • Autophagy Antibodies Anti-GABARAPL2 Antibody CAB7782
  • Autophagy Antibodies Anti-GABARAPL2 Antibody CAB7782
  • Autophagy Antibodies Anti-GABARAPL2 Antibody CAB7782
€139 - €419
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Description

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Antibody Name:Anti-GABARAPL2 Antibody
Antibody SKU:CAB7782
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKWM FKED HSLE HRCV ESAK IRAK YPDR VPVI VEKV SGSQ IVDI DKRK YLVP SDIT VAQF MWII RKRI QLPS EKAI FLFV DKTV PQSS LTMG QLYE KEKD EDGF LYVA YSGE NTFG F
Gene ID:11345
Uniprot:P60520
Cellular Location:Cytoplasmic vesicle, Golgi apparatus, autophagosome
Calculated MW:13kDa
Observed MW:14kDa
Synonyms:GABARAPL2, ATG8, ATG8C, GATE-16, GATE16, GEF-2, GEF2
Background:
UniProt Protein Function:GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR.
UniProt Protein Details:

Protein type:Vesicle; Microtubule-binding; Ubiquitin-like modifier; Autophagy; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: autophagic vacuole; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; intracellular

Molecular Function:ATPase binding; beta-tubulin binding; GABA receptor binding; protein binding; SNARE binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; intra-Golgi vesicle-mediated transport; macroautophagy; mitochondrion degradation; positive regulation of ATPase activity

UniProt Code:P60520
NCBI GenInfo Identifier:44888808
NCBI Gene ID:11345
NCBI Accession:P60520.1
UniProt Secondary Accession:P60520,O08765, Q6FG91, Q9DCP8, Q9UQF7,
UniProt Related Accession:P60520
Molecular Weight:13,667 Da
NCBI Full Name:Gamma-aminobutyric acid receptor-associated protein-like 2
NCBI Synonym Full Names:GABA type A receptor associated protein like 2
NCBI Official Symbol:GABARAPL2  
NCBI Official Synonym Symbols:ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16  
NCBI Protein Information:gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Protein Name:Gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Synonym Protein Names:GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; MAP1 light chain 3-related protein
Protein Family:Gamma-aminobutyric acid receptor-associated protein
UniProt Gene Name:GABARAPL2  
UniProt Entry Name:GBRL2_HUMAN
Anti-GABARAPL2 Antibody (CAB7782)Western blot analysis of extracts of mouse brain, using GABARAPL2 antibody (CAB7782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-GABARAPL2 Antibody (CAB7782)Immunohistochemistry of paraffin-embedded rat brain using GABARAPL2 antibody (CAB7782) at dilution of 1:200 (40x lens).
Anti-GABARAPL2 Antibody (CAB7782)Immunohistochemistry of paraffin-embedded mouse heart using GABARAPL2 antibody (CAB7782) at dilution of 1:200 (40x lens).
Anti-GABARAPL2 Antibody (CAB7782)Immunofluorescence analysis of A549 cells using GABARAPL2 antibody (CAB7782). Blue: DAPI for nuclear staining.

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