The GAD67/GAD1 Monoclonal Antibody (CAB1475) is a high-quality antibody developed for reliable detection and analysis of target proteins. These proteins are essential enzymes involved in the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA), which plays a crucial role in the central nervous system.Raised in rabbits, this polyclonal antibody is highly specific and reactive with human samples, making it ideal for use in various experimental applications, including Western blotting. By binding to GAD67 and GAD1 proteins, this antibody allows for the detection and analysis of these key enzymes in different cell types.The GAD67/GAD1 Antibody is particularly valuable in neuroscience research, as abnormalities in GABA synthesis have been linked to neurological disorders such as epilepsy, schizophrenia, and Parkinson's disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GAD67/GAD1 Monoclonal Antibody
SKU:
CAB1475
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1879
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Purification Method
Affinity purification
Gene ID
2571
RRID
AB_2861704
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GAD67/GAD1 Rabbit mAb (CAB1475) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using GAD67/GAD1 Rabbit mAb (CAB1475) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
