The Galactosidase alpha (GLA) Monoclonal Antibody (CAB5119) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in various research applications including Western blotting.Galactosidase alpha plays a crucial role in carbohydrate metabolism, specifically in the breakdown of certain sugars like lactose. Dysregulation of this enzyme has been implicated in various diseases, making it a target of interest for researchers in fields such as biochemistry, genetics, and medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Galactosidase alpha (GLA) Monoclonal Antibody
SKU:
CAB5119
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1213
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GALA, Galactosidase alpha (GLA)
Positive Sample:
Raji, Mouse brain, Rat brain
Cellular Localization:
Lysosome.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Purification Method
Affinity purification
Gene ID
2717
RRID
AB_2863453
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Galactosidase alpha (GLA) (GLA) Rabbit mAb (CAB5119) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
