The ENO2 Antibody (CAB12341) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. RRID AB_2759186 Gene ID 2026 Swiss Prot Synonym NSE; HEL-S-279; ENO2
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ENO2 Antibody
SKU:
CAB12341
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NSE, HEL-S-279, ENO2
Positive Sample:
SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. RRID AB_2759186 Gene ID 2026 Swiss Prot Synonym NSE; HEL-S-279; ENO2
Purification Method:
Affinity purification
Gene ID:
2026
RRID:
AB_2759186
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ENO2 Rabbit pAb (CAB12341) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using ENO2 Rabbit pAb (CAB12341) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Raji cells using ENO2 Rabbit pAb (CAB12341) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg ENO2 antibody (CAB12341). Western blot was performed from the immunoprecipitate using ENO2 antibody (CAB12341) at a dilution of 1:1000.
