The Pepsinogen II Antibody (CAB2788) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbits, offers high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the Gastricsin protein, researchers can accurately detect and analyze its presence in various cell types, making it an essential component for investigations in the fields of gastrointestinal physiology and pathology.Gastricsin, also known as pepsinogen C, plays a crucial role in the digestive process by breaking down proteins into smaller peptides.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Pepsinogen II Antibody
SKU:
CAB2788
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PEPC, PGII, Pepsinogen II (PG II)
Positive Sample:
Mouse liver, Mouse heart, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
42kDa
Observed MW:
45-50kDa
This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
Purification Method
Affinity purification
Gene ID
5225
RRID
AB_2764630
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Pepsinogen II (PG II) Rabbit pAb (CAB2788) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
