General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit
The General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit from AssayGenie is a reliable and accurate tool for quantifying cAMP levels in a variety of sample types, including serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, allowing for precise measurements of cAMP concentrations in biological samples. cAMP is a critical secondary messenger molecule involved in various cellular processes, such as signal transduction, gene expression, and metabolism. Dysregulation of cAMP signaling has been linked to numerous diseases, including cancer, diabetes, and neurological disorders.
By measuring cAMP levels in biological samples, researchers can gain valuable insight into the underlying mechanisms of these diseases and potentially identify new therapeutic targets. With easy-to-follow protocols and quick assay procedures, the General cAMP ELISA Kit is suitable for both experienced researchers and beginners in the field. Trust AssayGenie's ELISA kit to provide accurate and reliable results for your cAMP research needs. Order yours today and take your research to the next level.
Product Name:
General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
