The GNB2 Monoclonal Antibody (CAB9643) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By binding specifically to the GNB2 protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an ideal choice for studies in molecular biology and cell signaling research.GNB2 functions as a guanine nucleotide-binding protein that plays a crucial role in mediating the effects of various signaling molecules within the cell.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GNB2 Monoclonal Antibody
SKU:
CAB9643
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1675
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SSS4, HG2C1, NEDHYDF, GNB2
Positive Sample:
HeLa, NIH/3T3, Jurkat, SH-SY5Y, Rat brain
Cellular Localization:
Cytoplasm, Perinuclear Region, Cell Membrane.
Calculated MW:
37kDa
Observed MW:
35kDa
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR.
Purification Method
Affinity purification
Gene ID
2783
RRID
AB_2863746
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNB2 Rabbit mAb (CAB9643) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using GNB2 Rabbit mAb (CAB9643) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
