The GNE Polyclonal Antibody (CAB8570) is a valuable tool for researchers studying the CD300A protein. CD300A is a cell surface molecule that plays a key role in immune regulation, particularly in inhibiting immune responses. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.By binding to the CD300A protein, this antibody enables the detection and analysis of CD300A in various cell types, making it an ideal choice for studies in immunology and cancer research. CD300A is also known as an immune inhibitory receptor, and its function in immune homeostasis is crucial for regulating inflammation and inhibiting allergic reactions.
Research into CD300A is important for understanding its role in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. By studying CD300A, researchers can gain insights into immune response modulation and potentially develop therapies that target or modulate immune function in these disease contexts. The GNE Polyclonal Antibody (CAB8570) is a valuable tool for these research endeavors.
Product Name:
GNE Rabbit Polyclonal Antibody
SKU:
CAB8570
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GNE (NP_001121699.1).
Sequence:
METY GYLQ RESC FQGP HELY FKNL SKRN KQIM EKNG NNRK LRVC VATC NRAD YSKL APIM FGIK TEPE FFEL DVVV LGSH LIDD YGNT YRMI EQDD FDIN TRLH TIVR GEDE AAMV ESVG LALV KLPD VLNR LKPD IMIV HGDR FDAL AL
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100
Synonyms:
NM; DMRV; IBM2; Uae1; GLCNE; GNE
Positive Sample:
LO2,Jurkat,HeLa,mouse liver,rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10020
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using GNE Rabbit pAb (CAB8570) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
