The GNGT1 Antibody (CAB15675) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot assays.GNGT1, also known as G protein gamma transducin 1, plays a key role in signal transduction pathways within cells, making it a target of interest in studies related to cell signaling, neuronal development, and sensory perception.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
GNGT1 Antibody
SKU:
CAB15675
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GNG1, HG3G1, GNGT1
Positive Sample:
Mouse eye, Rat eye
Cellular Localization:
Cell Membrane, Cytoplasmic Side, Lipid-Anchor.
Calculated MW:
8kDa
Observed MW:
10kDa
This gene encodes the gamma subunit of transducin, a guanine nucleotide-binding protein (G protein) that is found in rod outer segments. Transducin, also known as GMPase, mediates the activation of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase by rhodopsin.
Purification Method
Affinity purification
Gene ID
2792
RRID
AB_2763084
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GNGT1 Rabbit pAb (CAB15675) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded rat eye using GNGT1 Rabbit pAb (CAB15675) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
