The GPR124 Polyclonal Antibody (PACO19729) is a valuable tool for researchers studying GPR124, a crucial protein involved in regulating blood-brain barrier integrity and angiogenesis. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.GPR124, also known as the adhesion G protein-coupled receptor, plays a critical role in mediating signaling pathways that control vascular development and maintenance of the blood-brain barrier. Dysregulation of GPR124 has been implicated in a variety of neurological disorders, making it a key target for research into conditions such as stroke, Alzheimer's disease, and cerebral cavernous malformations.
By using the GPR124 Polyclonal Antibody, researchers can accurately detect and analyze GPR124 expression in various cell types, aiding in the study of angiogenesis and blood-brain barrier function. This antibody is an essential tool for advancing our understanding of the role that GPR124 plays in health and disease, and may lead to the development of new therapeutic interventions for neurological disorders.
Antibody Name:
GPR124 Antibody (PACO19729)
Antibody SKU:
PACO19729
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human GPR124
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PACO19729(GPR124 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO19729(GPR124 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Tumor endothelial markers (TEMs) are abundantly expressed in the blood vessels of human solid tumors during angiogenesis and neoangiogensis. These include TEM1 (endosialin), TEM5 (G-protein coupled receptor 124) and TEM7 (plexin domain containing 1). TEMs are associated with the cell surface membrane at low levels in normal human and mouse tissues. TEM5 is a seven-pass transmembrane receptor, whereas TEM1, TEM7 and TEM8 span the membrane once. TEM5 expression is elevated during tumor angiogenesis and neoangiogenesis. TEM7 is highly expressed in tumor endothelium and neurons. Therefore, TEM5 and TEM7 may be suitable targets for the development of antiangiogenic therapies.
Synonyms:
G protein-coupled receptor 124
UniProt Protein Function:
GPR124: Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: GPCR, family 2; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 8p11.23Cellular Component: cell surface; integral to membrane; plasma membraneMolecular Function: G-protein coupled receptor activityBiological Process: G-protein coupled receptor protein signaling pathway; regulation of chemotaxis; central nervous system development; endothelial cell migration; sprouting angiogenesis; regulation of angiogenesis
