The GRB2 Monoclonal Antibody (CAB19059) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2862552 Gene ID 2885 Swiss Prot Synonym ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2; GRB2
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GRB2 Monoclonal Antibody
SKU:
CAB19059
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0430
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:200 - 1:800
IF
/
ICC
1:100 - 1:800
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
MCF7, 293T, HL-60, HeLa, NIH/3T3, Mouse brain, Mouse kidney, Rat spleen, Rat brain
Cellular Localization:
Cytoplasm, Endosome, Golgi Apparatus, Nucleus.
Calculated MW:
25kDa
Observed MW:
25kDa
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2862552 Gene ID 2885 Swiss Prot Synonym ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2; GRB2
Purification Method:
Affinity purification
Gene ID:
2885
RRID:
AB_2862552
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GRB2 Rabbit mAb (CAB19059) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GRB2 Rabbit mAb (CAB19059) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using GRB2 Rabbit mAb (CAB19059) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells using GRB2 Rabbit mAb (CAB19059,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
