The GSDMD Antibody (CAB18281) is a high-quality antibody developed for reliable detection and analysis of target proteins. Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GSDMD Antibody
SKU:
CAB18281
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF/ICC
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DF5L, DFNA5L, FKSG10, GSDMDC1, GSDMD
Positive Sample:
THP-1 treated with PMA and LPS, THP-1 treated with PMA, THP-1
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
79792
RRID
AB_2862055
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GSDMD Rabbit pAb (CAB18281) at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA(80 nM)at 37 ℃ for 24 hours, LPS(5 μg/mL) at 37 ℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
