The Glutathione (GSH) ELISA Kit is a specialized assay designed for the accurate measurement of glutathione levels in biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.Glutathione is a powerful antioxidant that plays a critical role in protecting cells from oxidative damage and maintaining cellular health. Imbalances in glutathione levels have been linked to various diseases such as cancer, liver disease, and neurodegenerative disorders, highlighting its importance as a potential biomarker for understanding and treating these conditions.
With its advanced technology and ease of use, the GSH ELISA Kit is a valuable tool for researchers seeking to investigate the role of glutathione in health and disease, as well as for developing new therapeutic strategies targeted at restoring glutathione homeostasis. Order your kit today and unlock the potential of glutathione research in your laboratory.
Product Name:
GSH (Glutathione) ELISA Kit
SKU:
UNES00017
Target:
GSH (Glutathione) (UNES00017)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 μg/mL
Detection range:
1.56-100 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GSH. During the reaction, GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
87-98
87-102
Average (%)
100
93
94
1:4
Range (%)
93-107
84-97
85-98
Average (%)
101
91
91
1:8
Range (%)
90-103
87-103
88-101
Average (%)
98
94
94
1:16
Range (%)
94-108
83-99
85-97
Average (%)
102
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
88-101
96
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.06
13.81
43.81
5.19
13.6
42.4
Standard deviation
0.27
0.82
2.24
0.27
0.66
1.59
C V (%)
5.34
5.94
5.11
5.2
4.85
3.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GSH in samples. No significant cross-reactivity or interference between GSH and analogues was observed.
