The GTF2E2 Antibody (CAB8732) is a high-quality antibody developed for reliable detection and analysis of target proteins. The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. RRID AB_2769711 Gene ID 2961 Swiss Prot Synonym FE; TTD6; TF2E2; TFIIE-B; GTF2E2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GTF2E2 Antibody
SKU:
CAB8732
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FE, TTD6, TF2E2, TFIIE-B, GTF2E2
Positive Sample:
Raji, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. RRID AB_2769711 Gene ID 2961 Swiss Prot Synonym FE; TTD6; TF2E2; TFIIE-B; GTF2E2
Purification Method:
Affinity purification
Gene ID:
2961
RRID:
AB_2769711
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GTF2E2 Rabbit pAb (CAB8732) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
