The Guinea Pig Interferon-gamma (IFNγ) ELISA Kit is a powerful tool for the accurate quantification of IFNγ levels in guinea pig serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable resource for a variety of research applications.IFNγ is a key cytokine involved in the immune response, playing a critical role in antiviral and antibacterial defense mechanisms. Additionally, IFNγ is essential for regulating inflammation and immune cell activation, making it a vital biomarker in studying immune-related diseases such as infections, autoimmune disorders, and inflammatory conditions.
By utilizing the Guinea Pig IFNγ ELISA Kit, researchers can gain valuable insights into the role of IFNγ in guinea pig models, furthering our understanding of immune function and potential therapeutic targets. Get accurate and dependable results with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Guinea pig Interferon Gamma (IFNg) ELISA Kit
Product Code:
GPDL00010
Size:
96 Assays
Target:
IFNg
Synonyms:
IFN-G, IFG, IFI, INFr, IFN, Immune Interferon
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
