The H2AFZ Polyclonal Antibody (CAB12442) is a specialized tool for researchers studying the H2AFZ protein, which is a core histone variant involved in chromatin regulation and gene expression. This antibody, produced using rabbit immunization, demonstrates high specificity for human samples and has been rigorously validated for Western blot applications. By binding specifically to the H2AFZ protein, this antibody enables precise detection and analysis in a variety of cell types, making it an invaluable asset for studies in epigenetics, gene regulation, and cancer biology.
H2AFZ, a variant of histone H2A, plays a crucial role in modulating chromatin structure and gene transcription, thereby influencing cellular processes such as DNA repair and cell cycle progression. Dysregulation of H2AFZ has been implicated in various diseases, including cancer and developmental disorders, making it a compelling target for further investigation. By elucidating the functions and regulatory mechanisms of H2AFZ, researchers can gain valuable insights into the molecular basis of disease pathogenesis and potentially identify novel therapeutic strategies.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Purification Method:
Affinity purification
Gene ID:
3015
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Histone H2A.Z Rabbit pAb (CAB12442) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
