The HACE1 Antibody (CAB9593) is a high-quality antibody developed for reliable detection and analysis of target proteins. HACE1 is a HECT domain-containing E3 ubiquitin ligase that plays a role in ubiquitin-mediated protein degradation and is involved in various cellular processes, including cell cycle regulation and tumor suppression. This polyclonal antibody, produced in rabbits, is highly specific to human HACE1 and has been validated for use in Western blotting applications. By binding to HACE1, this antibody allows for the detection and analysis of HACE1 protein levels in different cell types, making it ideal for studies in cancer research and cellular biology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HACE1 Antibody
SKU:
CAB9593
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor.
Purification Method
Affinity purification
Gene ID
57531
RRID
AB_2769728
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HACE1 Rabbit pAb (CAB9593) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using HACE1 Rabbit pAb (CAB9593) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
