The HBD Antibody (CAB9220) is a high-quality antibody developed for reliable detection and analysis of target proteins. HBD, also known as beta-defensin, is a key component of the immune system, playing a critical role in antimicrobial defense. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. The HBD Polyclonal Antibody binds specifically to the HBD protein, enabling accurate detection and analysis in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
HBD Antibody
SKU:
CAB9220
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HBD
Positive Sample:
Mouse lung, Rat liver
Cellular Localization:
Blood Microparticle, Cytosol, Hemoglobin Complex.
Calculated MW:
16kDa
Observed MW:
13kDa
The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia.
Purification Method
Affinity purification
Gene ID
3045
RRID
AB_2769742
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HBD Rabbit pAb (CAB9220) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
