The HDAC2 Monoclonal Antibody (CAB19626) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. RRID AB_2862705 Gene ID 3066 Swiss Prot Synonym HD2; RPD3; YAF1; KDAC2; HDAC2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HDAC2 Monoclonal Antibody
SKU:
CAB19626
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2647
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HD2, RPD3, YAF1, KDAC2, HDAC2
Positive Sample:
HeLa, 293T, NIH/3T3, C6, Mouse liver
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
55kDa
Observed MW:
55kDa
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. RRID AB_2862705 Gene ID 3066 Swiss Prot Synonym HD2; RPD3; YAF1; KDAC2; HDAC2
Purification Method:
Affinity purification
Gene ID:
3066
RRID:
AB_2862705
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HDAC2 Rabbit mAb (CAB19626) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
