The HEC1/NDC80 Monoclonal Antibody (CAB2392) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, offers high specificity and sensitivity for detecting HEC1 in various research applications, such as immunofluorescence and immunohistochemistry.HEC1 is a key component of the kinetochore, a protein complex essential for proper chromosome segregation during cell division. Dysregulation of HEC1 has been linked to chromosome instability and cancer development, making it a promising target for cancer research and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HEC1/NDC80 Monoclonal Antibody
SKU:
CAB2392
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0741
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
Purification Method
Affinity purification
Gene ID
10403
RRID
AB_2862998
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HEC1/NDC80 Rabbit mAb (CAB2392) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3 s.
