The HFE Monoclonal Antibody (CAB0937) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in various applications such as Western blot and immunohistochemistry. By targeting the HFE protein, this antibody enables precise detection and analysis of HFE expression in a variety of cell types, making it an ideal choice for studies in the fields of molecular biology and metabolic disorders.The HFE protein, also known as the major histocompatibility complex class I-like protein, plays a crucial role in controlling iron absorption and storage within the body.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HFE Monoclonal Antibody
SKU:
CAB0937
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2554
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HH, HFE1, HLA-H, MVCD7, TFQTL2, HFE
Positive Sample:
HeLa, HT-29, A549
Cellular Localization:
Cell Membrane.
Calculated MW:
40kDa
Observed MW:
45kDa
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene.
Purification Method
Affinity purification
Gene ID
3077
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HFE Rabbit mAb (CAB0937) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
