The Hist1H2AH Polyclonal Antibody (PAC016461) is a valuable tool for researchers studying the histone variant Hist1H2AH. Hist1H2AH is a key player in chromatin remodeling and gene regulation processes, making this antibody essential for investigating epigenetic mechanisms.This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various experimental techniques such as Western blotting and immunofluorescence. By binding to Hist1H2AH, researchers can accurately detect and analyze this histone variant in different cell types, providing valuable insights into its role in gene expression and chromatin dynamics.
Hist1H2AH has been linked to biological processes such as DNA repair, transcriptional regulation, and genomic stability, underscoring its importance in cell physiology and disease development. By utilizing the Hist1H2AH Polyclonal Antibody, researchers can further unravel the complexities of epigenetic regulation and potentially identify new therapeutic targets for conditions related to chromatin dysregulation.
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO16461(HIST1H2AH Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-4: K562 cells, Raji cells, Hela cells, 293T cells, Primary antibody: PACO16461(HIST1H2AH Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO16461(HIST1H2AH Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33.
Synonyms:
histone cluster 1, H2ah
UniProt Protein Function:
HIST1H2AH: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Belongs to the histone H2A family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p21.33Cellular Component: nucleosome; nucleusMolecular Function: DNA binding; protein heterodimerization activity
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Jul 2008]
