The Histone H2B Antibody (PAC023763) is a valuable tool for researchers studying histone proteins and their role in epigenetic regulation. Histones are essential components of chromatin, playing a key role in the organization and packaging of DNA in the nucleus. This antibody, raised in rabbits, is highly specific for the histone H2B protein and has been validated for use in various applications, including Western blotting and immunofluorescence.Histone H2B is involved in the compaction of DNA into nucleosomes, which are the building blocks of chromatin structure. Changes in histone modification, such as acetylation or methylation, can impact gene expression and cellular function.
Understanding the role of histone H2B in epigenetic regulation is critical for unraveling the complexities of gene expression and cell differentiation.This antibody provides researchers with a reliable tool for detecting and analyzing histone H2B in a variety of cell types and experimental settings. Its high specificity and sensitivity make it an ideal choice for studies in epigenetics, chromatin biology, and cancer research. By targeting histone H2B, researchers can gain valuable insights into the mechanisms underlying gene regulation and potentially develop new therapeutic approaches for various diseases.
Synthesized peptide derived from internal of human Histone H2B.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from A549 cells, using Histone H2B antibody.
Immunofluorescence analysis of HeLa cells, using Histone H2B antibody.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Histone H2B antibody.
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:
Histone H2B type 1-H; H2B.j; H2B/j; HIST1H2BH; H2BFJ
UniProt Protein Function:
H2B1H: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: DNA bindingBiological Process: nucleosome assembly
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
