The HLA-DRA Polyclonal Antibody (CAB11790) is a valuable tool for research involving the HLA-DRA protein, a key player in the immune system's ability to distinguish between self and non-self antigens. This antibody is produced in rabbits and is highly specific and reactive with human samples, making it ideal for use in Western blot applications.HLA-DRA is a major histocompatibility complex (MHC) class II protein that plays a crucial role in presenting antigens to T cells, thereby activating the immune response.
It is involved in antigen presentation and is essential for the adaptive immune system's ability to mount a targeted response against pathogens.Researchers studying autoimmune diseases, organ transplantation, infectious diseases, and cancer will benefit from using the HLA-DRA Polyclonal Antibody in their experiments. By accurately detecting and analyzing the HLA-DRA protein, researchers can gain valuable insights into the immune response mechanisms and potentially develop new therapeutic strategies.
Product Name:
HLA-DRA Rabbit Polyclonal Antibody
SKU:
CAB11790
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-216 of human HLA-DRA (NP_061984.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
29kDa
Observed MW:
37kDa
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Purification Method:
Affinity purification
Gene ID:
3122
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HLA-DRA antibody (CAB11790) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
