The HMBS Antibody (CAB1777) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the HMBS protein, it enables accurate detection and analysis in a variety of cell types, making it an essential reagent for investigations in biochemistry and molecular biology.HMBS, also known as hydroxymethylbilane synthase, plays a vital role in the production of heme, an essential component of hemoglobin and other hemoproteins. Dysregulation of HMBS activity can lead to various porphyrias, disorders characterized by abnormal heme synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HMBS Antibody
SKU:
CAB1777
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UPS, PBGD, PORC, PBG-D, HMBS
Positive Sample:
HeLa, Raji, Mouse spleen
Cellular Localization:
Cytoplasm.
Calculated MW:
39kDa
Observed MW:
45kDa
This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method
Affinity purification
Gene ID
3145
RRID
AB_2763819
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HMBS Rabbit pAb (CAB1777) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
