The HMG20A Antibody (CAB7286) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting HMG20A in human samples, making it ideal for use in Western blotting and immunohistochemistry applications.HMG20A, also known as High Mobility Group 20A, plays a critical role in controlling gene transcription and chromatin structure, impacting various cellular processes such as differentiation, proliferation, and development. Dysregulation of HMG20A has been linked to several diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, highlighting its importance in biomedical research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HMG20A Antibody
SKU:
CAB7286
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HMGX1, HMGXB1, HMG20A
Positive Sample:
HeLa, 293F, Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
40kDa
Observed MW:
40kDa
Enables identical protein binding activity. Predicted to be involved in regulation of gene expression. Predicted to act upstream of or within negative regulation of neuron differentiation; negative regulation of protein sumoylation; and negative regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
10363
RRID
AB_2767827
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HMG20A Rabbit pAb (CAB7286) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using HMG20A Rabbit pAb (CAB7286).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
