The HMG20A Antibody (CAB7286) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables identical protein binding activity. Predicted to be involved in regulation of gene expression. Predicted to act upstream of or within negative regulation of neuron differentiation; negative regulation of protein sumoylation; and negative regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. RRID AB_2767827 Gene ID 10363 Swiss Prot Synonym HMGX1; HMGXB1; HMG20A
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HMG20A Antibody
SKU:
CAB7286
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IF
/
ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HMGX1, HMGXB1, HMG20A
Positive Sample:
HeLa, 293F, Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
40kDa
Observed MW:
40kDa
Enables identical protein binding activity. Predicted to be involved in regulation of gene expression. Predicted to act upstream of or within negative regulation of neuron differentiation; negative regulation of protein sumoylation; and negative regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. RRID AB_2767827 Gene ID 10363 Swiss Prot Synonym HMGX1; HMGXB1; HMG20A
Purification Method:
Affinity purification
Gene ID:
10363
RRID:
AB_2767827
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HMG20A Rabbit pAb (CAB7286) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A-549 cells using HMG20A Rabbit pAb (CAB7286).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
