The HMGB3 Antibody (CAB15064) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to HMGB3, this antibody enables accurate detection and analysis of the protein in various cell types, making it an essential asset for studies in molecular biology and cancer research.HMGB3 plays a key role in regulating gene expression, DNA repair, and chromatin structure, making it a significant target for research on cancer, inflammation, and other diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
HMGB3 Antibody
SKU:
CAB15064
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HMG4, HMG-4, HMG2A, HMG-2a, HMGB3
Positive Sample:
Rat thymus
Cellular Localization:
Chromosome, Cytoplasm, Nucleus.
Calculated MW:
23kDa
Observed MW:
25kDa
This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3149
RRID
AB_2761946
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat thymus, using HMGB3 Rabbit pAb (CAB15064) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
