The HNMT Antibody (CAB13499) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human HNMT and is validated for Western blot applications. By binding to the HNMT protein, this antibody enables the detection and analysis of HNMT in various cell types, making it an essential component for studies in allergy research, histamine metabolism, and neurological disorders.HNMT plays a crucial role in histamine degradation, regulating the levels of this important biogenic amine in the body. Dysregulation of HNMT function has been linked to various conditions, including allergies, asthma, and neurological disorders.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HNMT Antibody
SKU:
CAB13499
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Purification Method
Affinity purification
Gene ID
3176
RRID
AB_2760362
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HNMT Rabbit pAb (CAB13499) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 1 μg HNMT antibody (CAB13499). Western blot was performed from the immunoprecipitate using HNMT antibody (CAB13499) at a dilution of 1:1000.
