The HRP-conjugated β-Actin Mouse mAb (CABC043) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from mouse monoclonal sources, is optimized for use in applications such as Western blotting and immunofluorescence.With its high reactivity towards actin in various species, including human samples, this antibody allows for accurate and reliable detection and analysis of actin protein levels in different cell types and tissues. Its conjugation with horseradish peroxidase (HRP) further enhances its utility by providing a signal amplification system for enhanced sensitivity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
HRP-conjugated β-Actin Mouse mAb
SKU:
CABC043
Size:
50μL, 100μL
Reactivity:
Human, Mouse, Rat, Monkey
Clone Number:
AMC0001
Conjugate:
HRP
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:50000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRWS1, PS1TP5BP1, HRP-conjugated β-Actin
Positive Sample:
293T, HeLa, A549, A-431, COS-7, NIH/3T3, C2C12, Mouse brain, Mouse testis, Mouse kindey, C6, PC-12, Rat brain, Rat testis, 293T, HeLa, A549, A-431, COS-7, NIH/3T3, Mouse brain, Mouse testis, Mouse kidney, C6, PC-12, Rat brain, Rat testis
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Purification Method
Affinity purification
Gene ID
60
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (CABC043) at 1:2000- 1:640000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of various lysates using HRP-conjugated β-Actin Mouse mAb (CABC043) at 1:10000 dilution incubated overnight at 4℃. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
