The HRP-conjugated Goat anti-Human IgG (H+L) (CABS002) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in WB applications and has demonstrated reactivity against Human samples.
Product Name:
HRP-conjugated Goat anti-Human IgG (H+L)
SKU:
CABS002
Size:
100μL
Reactivity:
Human
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:1000 - 1:10000
Positive Sample:
Human IgG1, Human IgG2, Human IgG4
Observed MW:
25kDa,55kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769850
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer:0.01M Sodium Phosphate, 0.25M NaCl, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free),pH 7.6
Western blot analysis of lysates from Human IgG, using HRP Goat Anti-Human IgG (H+L) (CABS002) at 1:2000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of recombinant Human immunoglobulin using HRP Goat Anti-Human IgG (H+L) (CABS002) at 1:10000 dilution incubated overnight at 4℃. Lysates/proteins: 50ng - 100ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
